Ammonium sulfate precipitation of proteins protocol

The following protocol is for secreted proteins. Modify according to other soluble proteins The spin down cells Pour the supernatant into a flask or a glass Remove the small part to analyze the gel Add the desired amount of ammonium sulphate very slowly (in the form of a saturated liquid, or in powder form) Stir … Read more

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NA Agarose Gel Electrophoresis – How to Run a DNA Agarose Gel

50x TAETris 121gEDTA 50 mL 500mm (EDTA pH to pH 8.0 with NaOH first before adding)Acetic acid 28.55mLMake up to 500ml Make 1x TAE from 50x to water NA Agarose Gel Electrophoresis – How to Run a DNA Agarose Gel Pouring and Running agarose gel disposable gloves when handling agarose gels, such as the highly … Read more

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Cell transfection protocol (calcium phosphate method)

Based Maniatis. Solutions2x BBS50 mM BES280 mM NaCl1.5 mM Na2HP04 calcium chloride1M CaCl2 Cell transfection protocol (calcium phosphate method) No need to dish out the cells one day in advance before the actual transfection. The shape of complex DNA: DNA using 20ug and watered in 188uL water, add 62uL of 1M CaCl2, then add 250uL … Read more

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