Using tissue culture chamber
keep the door closed to prevent contamination from the outside into the room.
turn on the fan in the hood ~ 15 minutes before use.
wiping counters with ethanol before use.
fire bottles, metal boxes for length and pipets pasteur pipets before and after use.
when finished with the aspirator, the aspirations of some 5% bleach to wash out the hose.
wiping counters with ethanol when finished.
if 70% ethanol or 5% bleach full, refill them; if thermos full to empty fluid, changing it by adding bleach to a flask, rinse with water, and running water in the sink.