Detection and quantification of the toxic microalgae Karenia brevis using lab on a chip mRNA sequence-based amplification.

Now and again, the rapid proliferation of certain species of phytoplankton can give rise to Harmful Algal Blooms, which pose a serious threat to marine life and human health. Current methods of monitoring phytoplankton are limited by poor specificity or by the requirement to return samples to a highly resourced, centralised lab.

The Lab Card is a small, microfluidic cassette which, when used in tandem with a portable Lab Card Reader can be used to sensitively and specifically quantify harmful algae in the field, from nucleic acid extracts using RNA amplification; a sensitive and specific method for the enumeration of potentially any species based on their unique genetic signatures.

This study reports the culmination of work to develop a Lab Card-based genetic assay to quantify the harmful algae Karenia brevis using mRNA amplification by the Nucleic Acid Sequence Based Amplification (NASBA) method. K. brevis cells were quantified by amplification of the rbcL gene transcript in nucleic acid extracts of K. brevis cell samples.

Chicken C Reactive Protein (CRP) ELISA Kit
DLR-CRP-Ch-96T	DL Develop	96T	EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Chicken C Reactive Protein (CRP) in samples from serum, plasma or other biological fluids.

Human C Reactive Protein (CRP) ELISA Kit
DLR-CRP-Hu-48T	DL Develop	48T	EUR 385
Description: A sandwich quantitative ELISA assay kit for detection of Human C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates or other biological fluids.

Human C Reactive Protein (CRP) ELISA Kit
DLR-CRP-Hu-96T	DL Develop	96T	EUR 492
Description: A sandwich quantitative ELISA assay kit for detection of Human C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates or other biological fluids.

Mouse C Reactive Protein (CRP) ELISA Kit
DLR-CRP-Mu-48T	DL Develop	48T	EUR 489
Description: A sandwich quantitative ELISA assay kit for detection of Mouse C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse C Reactive Protein (CRP) ELISA Kit
DLR-CRP-Mu-96T	DL Develop	96T	EUR 635
Description: A sandwich quantitative ELISA assay kit for detection of Mouse C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine C Reactive Protein (CRP) ELISA Kit
DLR-CRP-p-48T	DL Develop	48T	EUR 547
Description: A sandwich quantitative ELISA assay kit for detection of Porcine C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine C Reactive Protein (CRP) ELISA Kit
DLR-CRP-p-96T	DL Develop	96T	EUR 715
Description: A sandwich quantitative ELISA assay kit for detection of Porcine C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat C Reactive Protein (CRP) ELISA Kit
DLR-CRP-Ra-48T	DL Develop	48T	EUR 426
Description: A sandwich quantitative ELISA assay kit for detection of Rat C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat C Reactive Protein (CRP) ELISA Kit
DLR-CRP-Ra-96T	DL Develop	96T	EUR 549
Description: A sandwich quantitative ELISA assay kit for detection of Rat C Reactive Protein (CRP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rabbit C Reactive Protein (CRP) ELISA Kit
DLR-CRP-Rb-48T	DL Develop	48T	EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit C Reactive Protein (CRP) in samples from serum, plasma or other biological fluids.

Rabbit C Reactive Protein (CRP) ELISA Kit
DLR-CRP-Rb-96T	DL Develop	96T	EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit C Reactive Protein (CRP) in samples from serum, plasma or other biological fluids.

Bovine C Reactive Protein (CRP) ELISA Kit
RD-CRP-b-48Tests	Reddot Biotech	48 Tests	EUR 555

Bovine C Reactive Protein (CRP) ELISA Kit
RD-CRP-b-96Tests	Reddot Biotech	96 Tests	EUR 771

Chicken C Reactive Protein (CRP) ELISA Kit
RD-CRP-Ch-48Tests	Reddot Biotech	48 Tests	EUR 511

Chicken C Reactive Protein (CRP) ELISA Kit
RD-CRP-Ch-96Tests	Reddot Biotech	96 Tests	EUR 709

Human C Reactive Protein (CRP) ELISA Kit
RD-CRP-Hu-48Tests	Reddot Biotech	48 Tests	EUR 372

Human C Reactive Protein (CRP) ELISA Kit
RD-CRP-Hu-96Tests	Reddot Biotech	96 Tests	EUR 510

Mouse C Reactive Protein (CRP) ELISA Kit
RD-CRP-Mu-48Tests	Reddot Biotech	48 Tests	EUR 489

Mouse C Reactive Protein (CRP) ELISA Kit
RD-CRP-Mu-96Tests	Reddot Biotech	96 Tests	EUR 677

Porcine C Reactive Protein (CRP) ELISA Kit
RD-CRP-p-48Tests	Reddot Biotech	48 Tests	EUR 555

Porcine C Reactive Protein (CRP) ELISA Kit
RD-CRP-p-96Tests	Reddot Biotech	96 Tests	EUR 771

Rat C Reactive Protein (CRP) ELISA Kit
RD-CRP-Ra-48Tests	Reddot Biotech	48 Tests	EUR 419

Rat C Reactive Protein (CRP) ELISA Kit
RD-CRP-Ra-96Tests	Reddot Biotech	96 Tests	EUR 577

Rabbit C Reactive Protein (CRP) ELISA Kit
RD-CRP-Rb-48Tests	Reddot Biotech	48 Tests	EUR 511

Rabbit C Reactive Protein (CRP) ELISA Kit
RD-CRP-Rb-96Tests	Reddot Biotech	96 Tests	EUR 709

Bovine C Reactive Protein (CRP) ELISA Kit
RDR-CRP-b-48Tests	Reddot Biotech	48 Tests	EUR 580

Bovine C Reactive Protein (CRP) ELISA Kit
RDR-CRP-b-96Tests	Reddot Biotech	96 Tests	EUR 807

Chicken C Reactive Protein (CRP) ELISA Kit
RDR-CRP-Ch-48Tests	Reddot Biotech	48 Tests	EUR 534

Chicken C Reactive Protein (CRP) ELISA Kit
RDR-CRP-Ch-96Tests	Reddot Biotech	96 Tests	EUR 742

Human C Reactive Protein (CRP) ELISA Kit
RDR-CRP-Hu-48Tests	Reddot Biotech	48 Tests	EUR 388

Human C Reactive Protein (CRP) ELISA Kit
RDR-CRP-Hu-96Tests	Reddot Biotech	96 Tests	EUR 533

Mouse C Reactive Protein (CRP) ELISA Kit
RDR-CRP-Mu-48Tests	Reddot Biotech	48 Tests	EUR 511

Mouse C Reactive Protein (CRP) ELISA Kit
RDR-CRP-Mu-96Tests	Reddot Biotech	96 Tests	EUR 709

Porcine C Reactive Protein (CRP) ELISA Kit
RDR-CRP-p-48Tests	Reddot Biotech	48 Tests	EUR 580

Porcine C Reactive Protein (CRP) ELISA Kit
RDR-CRP-p-96Tests	Reddot Biotech	96 Tests	EUR 807

Rat C Reactive Protein (CRP) ELISA Kit
RDR-CRP-Ra-48Tests	Reddot Biotech	48 Tests	EUR 437

Rat C Reactive Protein (CRP) ELISA Kit
RDR-CRP-Ra-96Tests	Reddot Biotech	96 Tests	EUR 603

Rabbit C Reactive Protein (CRP) ELISA Kit
RDR-CRP-Rb-48Tests	Reddot Biotech	48 Tests	EUR 534

Rabbit C Reactive Protein (CRP) ELISA Kit
RDR-CRP-Rb-96Tests	Reddot Biotech	96 Tests	EUR 742

Guinea pig C Reactive Protein (CRP) ELISA Kit
DLR-CRP-Gu-48T	DL Develop	48T	EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Guinea pig C Reactive Protein (CRP) in samples from serum, plasma or other biological fluids.

Guinea pig C Reactive Protein (CRP) ELISA Kit
DLR-CRP-Gu-96T	DL Develop	96T	EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Guinea pig C Reactive Protein (CRP) in samples from serum, plasma or other biological fluids.

Guinea pig C Reactive Protein (CRP) ELISA Kit
RD-CRP-Gu-48Tests	Reddot Biotech	48 Tests	EUR 533

Guinea pig C Reactive Protein (CRP) ELISA Kit
RD-CRP-Gu-96Tests	Reddot Biotech	96 Tests	EUR 740

Guinea pig C Reactive Protein (CRP) ELISA Kit
RDR-CRP-Gu-48Tests	Reddot Biotech	48 Tests	EUR 557

Guinea pig C Reactive Protein (CRP) ELISA Kit
RDR-CRP-Gu-96Tests	Reddot Biotech	96 Tests	EUR 774

TruStrip RDT Dog C-reactive Protein (CRP) Rapid Test cards, 10 tests/pack
CRP-RDT-D10	Alpha Diagnostics	1 pack	EUR 171

TruStrip RDT Human C-reactive Protein (CRP) Rapid Test cards, 10 tests/pack
CRP-RDT-H10	Alpha Diagnostics	1 pack	EUR 171

TruStrip RDT Human C-reactive Protein (CRP) Rapid Test cards, 25 tests/pack
CRP-RDT-H25	Alpha Diagnostics	1 pack	EUR 293

TruStrip RDT Monkey C-reactive Protein (CRP) Rapid Test cards, 10 tests/pack
CRP-RDT-M10	Alpha Diagnostics	1 pack	EUR 171

TruStrip RDT Monkey C-reactive Protein (CRP) Rapid Test cards, 25 tests/pack
CRP-RDT-M25	Alpha Diagnostics	1 pack	EUR 293

TruStrip RDT Rat C-reactive Protein (CRP) Rapid Test cards, 10 tests/pack
CRP-RDT-R10	Alpha Diagnostics	1 pack	EUR 171

TruStrip RDT Rat C-reactive Protein (CRP) Rapid Test cards, 25 tests/pack
CRP-RDT-R25	Alpha Diagnostics	1 pack	EUR 293

CRP c-Reactive Protein (19-224 a.a) Human Recombinant Protein
PROTP02741-2	BosterBio	Regular: 20ug	EUR 317
Description: CRP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids (19-224 a.a.) and having a molecular mass of 23.2kDa.;CRP is purified by proprietary chromatographic techniques.

CRP/ Rat CRP ELISA Kit
ELA-E0829r	Lifescience Market	96 Tests	EUR 886

Crp/ Rat Crp ELISA Kit
ELI-02799r	Lifescience Market	96 Tests	EUR 886

Canine CRP(CRP) ELISA Kit
QY-E70170	Qayee Biotechnology	96T	EUR 426

CRP protein
80R-4350	Fitzgerald	50 ug	EUR 349
Description: Purified Recombinant CRP protein (His tagged)

CRP antibody
70R-4527	Fitzgerald	50 ug	EUR 467
Description: Rabbit polyclonal CRP antibody raised against the N terminal of CRP

CRP Antiboday
70R-51961	Fitzgerald	100 ug	EUR 197
Description: Purified Rabbit CRP antibody

CRP Antibody
BF0116	Affbiotech	200ul	EUR 376
Description: CRP antibody detects endogenous levels of total CRP.

CRP Antibody
ABD6027	Lifescience Market	100 ug	EUR 438

anti-CRP
YF-PA27200	Abfrontier	50 ug	EUR 363
Description: Mouse polyclonal to CRP

CRP protein
30C-CP1000U	Fitzgerald	1 mg	EUR 349
Description: Purified native Human CRP protein

CRP protein
30R-2081	Fitzgerald	100 ug	EUR 322
Description: Recombinant human CRP protein

CRP protein
30R-2389	Fitzgerald	250 ug	EUR 152
Description: Purified recombinant Human CRP protein

CRP protein
30R-2740	Fitzgerald	10 ug	EUR 341
Description: Purified recombinant Human CRP protein

CRP protein
30R-2984	Fitzgerald	1 mg	EUR 380
Description: Purified recombinant Human CRP protein

CRP Protein
30R-3412	Fitzgerald	1 mg	EUR 300
Description: Human C-reactive protein

CRP protein
30R-AC001x	Fitzgerald	10 mg	EUR 457
Description: Highly purifed Human CRP protein

CRP protein
30R-AC067	Fitzgerald	1 mg	EUR 448
Description: Purified native Human CRP protein

CRP protein
30-1092	Fitzgerald	1 mg	EUR 393
Description: Purified recombinant Human CRP protein

CRP protein
30-1094	Fitzgerald	100 ug	EUR 155
Description: Purified native Human CRP protein

CRP protein
30-1906	Fitzgerald	1 mg	EUR 336
Description: Native human CRP protein

CRP protein
30-AC05	Fitzgerald	5 mg	EUR 277
Description: Highly purified Human CRP protein

CRP protein
30-AC07	Fitzgerald	5 mg	EUR 1029
Description: Purified Recombinant CRP protein

CRP protein
30-AC10	Fitzgerald	5 mg	EUR 250
Description: Partially purified Human CRP protein

CRP antibody
20C-CR2015S	Fitzgerald	1 ml	EUR 133
Description: Sheep polyclonal CRP antibody

CRP antibody
20C-CR2015SP	Fitzgerald	1 ml	EUR 177
Description: Sheep polyclonal CRP antibody

CRP antibody
70R-10663	Fitzgerald	1 ml	EUR 484
Description: Rabbit polyclonal CRP antibody

CRP antibody
70-B9007GA00-A0	Fitzgerald	5 mg	EUR 241
Description: Goat polyclonal Human CRP antibody

CRP Antibody
6690-100	Biovision		EUR 338

CRP Antibody
6690-30T	Biovision		EUR 146

CRP antibody
70R-13710	Fitzgerald	100 ug	EUR 322
Description: Affinity purified Sheep polyclonal CRP antibody

CRP antibody
10R-3002	Fitzgerald	100 ug	EUR 265
Description: Mouse monoclonal CRP antibody

CRP antibody
10R-C189a	Fitzgerald	1 mg	EUR 284
Description: Mouse monoclonal CRP antibody

CRP antibody
10R-C189b	Fitzgerald	1 mg	EUR 399
Description: Mouse monoclonal CRP antibody

CRP antibody
10C-CR2015M1	Fitzgerald	1 mg	EUR 168
Description: Mouse monoclonal CRP antibody

CRP antibody
10C-CR2015M5	Fitzgerald	1 mg	EUR 165
Description: Mouse monoclonal CRP antibody

CRP antibody
10C-CR2015M6	Fitzgerald	1 mg	EUR 165
Description: Mouse monoclonal CRP antibody

CRP antibody
10R-10264	Fitzgerald	50 ug	EUR 241
Description: Mouse monoclonal CRP antibody

CRP antibody
10R-1042	Fitzgerald	100 ul	EUR 349
Description: Mouse monoclonal CRP antibody

CRP antibody
10-2276	Fitzgerald	100 ug	EUR 221
Description: Recombinant Fab monoclonal CRP antibody

CRP antibody
10-2349	Fitzgerald	1 mg	EUR 349
Description: Mouse monoclonal CRP antibody

CRP antibody
10-2350	Fitzgerald	1 mg	EUR 349
Description: Mouse monoclonal CRP antibody

CRP antibody
10-2703	Fitzgerald	1 mg	EUR 165
Description: Mouse Monoclonal CRP antibody

CRP antibody
10-7893	Fitzgerald	1 mg	EUR 392
Description: Mouse monoclonal CRP antibody

CRP antibody
10-7894	Fitzgerald	1 mg	EUR 403
Description: Mouse monoclonal CRP antibody

CRP antibody
10-C189A	Fitzgerald	1 mg	EUR 245
Description: Mouse monoclonal CRP antibody

CRP antibody
10-C189B	Fitzgerald	1 mg	EUR 420
Description: Mouse monoclonal CRP antibody

CRP antibody
10-C33A	Fitzgerald	1 mg	EUR 238
Description: Mouse monoclonal CRP antibody

CRP antibody
10-C33AS	Fitzgerald	1 mg	EUR 235
Description: Mouse monoclonal CRP antibody

CRP antibody
10-C33B	Fitzgerald	1 mg	EUR 662
Description: Mouse monoclonal CRP antibody

CRP antibody
10-C33C	Fitzgerald	1 mg	EUR 245
Description: Mouse monoclonal CRP antibody

CRP antibody
20-1002	Fitzgerald	1 ml	EUR 111
Description: Goat polyclonal Human CRP antibody

CRP antibody
20-1003	Fitzgerald	1 ml	EUR 133
Description: Rabbit polyclonal Human CRP antibody

CRP antibody
20-1262	Fitzgerald	1 ml	EUR 647
Description: Sheep polyclonal CRP antibody

CRP antibody
20-B9007G000-W0	Fitzgerald	10 ml	EUR 116
Description: Goat polyclonal Human CRP antibody

CRP antibody
20-B9007GD00-D0	Fitzgerald	5 mg	EUR 138
Description: Goat polyclonal Human CRP antibody

CRP antibody
20-S3903GND1-D0	Fitzgerald	1 ml	EUR 133
Description: Polyclonal CRP antibody

CRP antibody
20-S3903GND9-D0	Fitzgerald	10 ml	EUR 127
Description: Goat polyclonal Human CRP antibody

CRP antibody
20-S3911G000-S4	Fitzgerald	10 ml	EUR 116
Description: Goat polyclonal Human CRP antibody

CRP antibody
20-S3911G000-V0	Fitzgerald	10 ml	EUR 133
Description: Goat polyclonal Human CRP antibody

CRP antibody
20-S3911G001-V0	Fitzgerald	10 ml	EUR 133
Description: Goat polyclonal Human CRP antibody

CRP antibody
20-S6011G000-S4	Fitzgerald	10 ml	EUR 138
Description: Goat polyclonal CRP antibody

CRP antibody
10R-8426	Fitzgerald	100 ul	EUR 393
Description: Mouse monoclonal CRP antibody

CRP antibody
10-1004	Fitzgerald	1 mg	EUR 457
Description: Mouse monoclonal CRP antibody

CRP Antibody
DF6027	Affbiotech	200ul	EUR 304
Description: CRP Antibody detects endogenous levels of total CRP.

CRP Antibody
1-CSB-PA06079A0Rb	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against CRP. Recognizes CRP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

CRP Antibody
1-CSB-PA181901	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against CRP. Recognizes CRP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:100-1:300

crp Antibody
1-CSB-PA359785LA01ENV	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against crp. Recognizes crp from Escherichia coli. This antibody is Unconjugated. Tested in the following application: ELISA

A novel enzyme dehydration and preservation method was combined with a pre-existing reagent Gelification method to prepare fully preserved Lab Cards with a shelf-life of at least six weeks prior to use. Using an internal control (IC), the Lab Card-based rbcL NASBA was demonstrated for the quantification of K. brevis from cell extracts containing between 50 and 5000 cells. This is the first demonstration of quantitation of K. brevis using IC-NASBA on a Lab Card.