Co-Immunoprecipitation (co-IP) Protocol on How to Find Protein-Protein Interactions

washing buffer20mm Tris0.2% Tritonor1M Tris 1mL20% Triton 500uLwater up to 50 ml elution bufferthe same solution as washing buffer, but with 4% SDS (may also PI if necessary, but it may not be necessary, because the other protein has been purified out) Spin down cell debris in right ultracentrifuge at 100,000 x g for 10 … Read more

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