Cell transfection protocol (calcium phosphate method)

Based Maniatis. Solutions2x BBS50 mM BES280 mM NaCl1.5 mM Na2HP04 calcium chloride1M CaCl2 Cell transfection protocol (calcium phosphate method) No need to dish out the cells one day in advance before the actual transfection. The shape of complex DNA: DNA using 20ug and watered in 188uL water, add 62uL of 1M CaCl2, then add 250uL … Read more

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